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6 Calculate the free energy yield available to two types of nitrate-reducing microorganisms those converting nitrate to nitrogen gas (N2; eg, Pseudomonas) and those converting nitrate to ammonia (E coli) Assume that the electron donor for each reaction is carbohydrate (CH2O) To carry out the calculation, use Box 34 (see nal preferred approach for calculating biochemical free energy yield) and Figure 39 Assume Eo values for the electron donor and two electron acceptors are as follow: CO2/CH2O, 043 V; NO3/NH3, +036 V; NO3/N2, +075 V 7 Regarding your answer to question 6, given the signi cantly smaller free energy yield when nitrate is reduced to ammonia, why would E coli use dissimilatory reduction of nitrate to ammonia (Hint: consider the stoichiometry, especially the number of electrons accepted For additional, background, see Section 74.

During my writing Escape From Excel Hell I have had the good fortune of crossing paths with Howard Dammond, a colleague I had known only peripherally After I spoke to him about Escape From Excel Hell, all that changed Throughout the course of my writing, we engaged in a wonderful dialog, making Howard an incredible resource who substantially enhanced the quality of this book In writing this book I have benefited from the many thousands of online questions and answers The people both asking questions and providing answers have allowed me to better discern what is important in the minds of people, brilliant insights, and clever techniques To both communities of users and experts alike, I am grateful for the open dialogue you have placed into numerous public forums.

2 . .0.6 4 . .0.6 2 . .3.4

) 8 Note that the stoichiometric redox equations on the right-hand side of Table 39 are written with carbohydrate (CH2O) as the electron donor (A) Prepare balanced redox equations for metabolism of the fuel component, toluene (C7H8), under aerobic, denitrifying, iron-reducing, sulfate-reducing, and methanogenic conditions Be sure to account rst for the electrons transferred in the redox reactions, then use mass, charge, water, and protons to complete the balancing (see Box 34) (B) In both sets of equations (glucose and toluene) some of these metabolic processes consume H+ What geochemical impact would this have on eld sites where these processes occur 9 A deep-sea hydrothermal vent emits dissolved concentrations of hydrogen gas, methane, hydrogen sul de, and Fe2+ into aerobic waters (A) Write balanced stoichiometric reactions between oxygen (electron acceptor) and each of the four electron donors (B) Using the thermodynamic tools explained in Box 3.

4 and Figure 39, calculate the free energy yield for each reaction to rank the four potential electron donors from most to least physiologically bene cial (C) Regarding the microbiology of oceanic hydrothermal vent sites, what major factors other than those treated in (B) above are likely to determine processes and populations likely to ourish in vent communities (D) How would you prove that each of these potentially useful energy sources was actually used by microorganisms in situ.

I would also like to thank the following people, many of whom have contributed ideas in public and online forums (appearing in order of first name): Chip Pearon, Dana DeLouis, Dann Stayskal, Dave Peterson, David Billigmeier, David Geen, David McRitchie, Debra Dalgleish, Dermot Balson, Don Guillett, Gord Dibben, Harlan Grove, Harry Butler, JE McGimpsey, John Picard, John Tassopoulos, John Walkenbach, Jon Peltier, Kevin Gordon, Martin Los, Mike Alexander, Norman Harker, Norman Jones, Patrick O Beirne, Peo Sjoblom, Steve Newbern, and Tom Ogilvy I feel that it s a privilege to work with Greg Croy and Susan Christophersen, and the whole team at Wiley Their professionalism and dedication truly typifies best practices I want to thank John Walkenbach for his feedback during the early stages of writing, which helped shape the book Bill Good is an amazingly talented individual who knows how to think outside and in the box.

(for x = 0, L)

Chatzi and Kiparissides (1994)